Home > Sandwich ELISA Kits > Human Lysosomal Associated Membrane Protein 2 (LAMP2) ELISA Kit

Product Name:

Human Lysosomal Associated Membrane Protein 2 (LAMP2) ELISA Kit

Cat.#:

JL52514

Regulatory Status:

RUO

Alternative:

CD107-B; CD107b; LAMP-2C; LAMPB; LGP96; Lysosome-associated membrane glycoprotein 2; CD107 antigen-like family member B

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

62.5-4000pg/mL

Sensitivity:

31.6pg/mL

Specificity:

This kit recognizes Human LAMP2 in samples. No significant cross-reactivity or interference between Human LAMP2 and analogues was observed.

Sample Type:

Serum, plasma and other biological fluids

Assay Procedure:

Research Area:

Cardiovascular Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| LAMP2

Lysosome-associated membrane protein 2 (LAMP2), also known as CD107b (Cluster of Differentiation 107b) and Mac-3. Its protein, LAMP2, is one of the lysosome-associated membrane glycoproteins.The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of the gene produces three variants - LAMP-2A, LAMP-2B and LAMP-2C.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	4000	2000	1000	500	250	125	62.5	0
OD	2.28	1.63	1.04	0.69	0.45	0.3	0.22	0.09
Corrected OD	2.19	1.54	0.95	0.6	0.36	0.21	0.13	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human LAMP2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102

| Linearity

Samples were spiked with high concentrations of Human LAMP2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	94	97